|
| Status |
Public on Jan 01, 2014 |
| Title |
KO wounded skin 24 hr |
| Sample type |
RNA |
| |
|
| Source name |
KO_wounded_skin
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL6
genotype/variation: Med1 KO; MED1 fl/fl K14 driven Cre
age: 8 wks
hair cycling stage: telogen
tissue: skin
sample group: wounded
time after wound: 24 hr
|
| Treatment protocol |
Med1 KO mice and their littermate control mice received two full-thickness wounds with a 6-mm biopsy punch on the telogen skin. Med1 KO mice showed both telogen and pigmented anagen skin because of hair cycling defects, In contrast, control skin was in telogen in whole area at 8 wk of age. We made wounds on telogen skin. The telogen wounded skin was excised by using 8-mm biopsy punch after 24 hr of wound preparation. Non-wounded telogen skin was also excised from the same mouse as a control.
|
| Growth protocol |
Coactivator MED1 is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from wounded or non wounded skin by using combination of RNAzol and RNAeasy RNA purification kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
|
| Scan protocol |
Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
|
| Description |
KO wound 24hr
|
| Data processing |
data were normalized by using quantile method in Genome studio V2011.1
|
| |
|
| Submission date |
Sep 06, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Yuko Oda |
| E-mail(s) |
yuko.oda@ucsf.edu
|
| Phone |
4152692370
|
| Organization name |
VA Medical Center UCSF
|
| Department |
Endocrine Research
|
| Street address |
111N-MB 360 1700 Owens St, Suite 360
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE50670 |
Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [skin wound] |
| GSE50673 |
Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury. |
|
