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Sample GSM1226049 Query DataSets for GSM1226049
Status Public on Jan 01, 2014
Title Control (WT) rep4
Sample type RNA
 
Source name WT_skin
Organism Mus musculus
Characteristics strain background: C57BL6
genotype/variation: control WT; MED1 fl/fl
age: 10 wks
tissue: whole skin excised from back
Treatment protocol Whole skin was exised from back skin and RNA was extracted from each mouse
Growth protocol Coactivator MED1 is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from whole skin by using combination of RNAzol and RNAeasy RNA purification kit (Qiagen)
Label biotin
Label protocol Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
 
Hybridization protocol Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
Scan protocol Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
Description WT4
Data processing data were normalized by using quantile method in Genome studio V2011.1
 
Submission date Sep 06, 2013
Last update date Jan 01, 2014
Contact name Yuko Oda
E-mail(s) yuko.oda@ucsf.edu
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL6885
Series (2)
GSE50671 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [10wk skin]
GSE50673 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury.

Data table header descriptions
ID_REF
VALUE quantile normalized using Genome Studio
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 103.5 0
ILMN_2707227 20.5 0.04511
ILMN_2896528 4564 0
ILMN_2721178 924.5 0
ILMN_1227723 436.7 0
ILMN_3033922 1386.3 0
ILMN_3092673 7375.7 0
ILMN_2730714 4878 0
ILMN_3162224 2227.3 0
ILMN_2816356 25.6 0.02506
ILMN_2808939 920.3 0
ILMN_2634564 345 0
ILMN_1216623 427.7 0
ILMN_1215157 867.2 0
ILMN_1228777 4.4 0.25188
ILMN_2737647 3 0.2995
ILMN_1216425 -3.9 0.61654
ILMN_2734484 546.7 0
ILMN_2652961 -3.4 0.59649
ILMN_2952292 77.9 0.00125

Total number of rows: 25697

Table truncated, full table size 594 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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