|
Status |
Public on Jan 01, 2014 |
Title |
Med1 knockout (KO) rep3 |
Sample type |
RNA |
|
|
Source name |
Med1 KO_skin
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL6 genotype/variation: Med1 KO; MED1 fl/fl K14 driven Cre age: 10 wks tissue: whole skin excised from back
|
Treatment protocol |
Whole skin was exised from back skin and RNA was extracted from each mouse
|
Growth protocol |
Coactivator MED1 is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from whole skin by using combination of RNAzol and RNAeasy RNA purification kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
|
Scan protocol |
Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
|
Description |
KO3
|
Data processing |
data were normalized by using quantile method in Genome studio V2011.1
|
|
|
Submission date |
Sep 06, 2013 |
Last update date |
Jan 01, 2014 |
Contact name |
Yuko Oda |
E-mail(s) |
yuko.oda@ucsf.edu
|
Phone |
4152692370
|
Organization name |
VA Medical Center UCSF
|
Department |
Endocrine Research
|
Street address |
111N-MB 360 1700 Owens St, Suite 360
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE50671 |
Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [10wk skin] |
GSE50673 |
Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury. |
|