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Sample GSM1226054 Query DataSets for GSM1226054
Status Public on Jan 01, 2014
Title Control (WT) keratinocyte rep1
Sample type RNA
 
Source name WT_10wk_keratinocyte
Organism Mus musculus
Characteristics strain background: C57BL6
genotype/variation: control WT; MED1 fl/fl
age: 10 wk
tissues: skin; epidermis
cell type: keratinocyte
Treatment protocol Keratinocytes were isolated from the skin accoding to the method previously described (Section 3.3 Nowarks JA Fuchs E Mol Biol. 2009 ; 482: 215–232). Briefly, exsided skin was incubated with 0.25% trypsin and epidermis was separated from dermis. Keratinocytes were then isolated from the epidermis.
Growth protocol Coactivator MED1 is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from keratinocytes using RNAeasy RNA purification kit (Qiagen).
Label biotin
Label protocol Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
 
Hybridization protocol Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
Scan protocol Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
Description WT1
Data processing data were normalized by using quantile method in Genome studio V2011.1
 
Submission date Sep 06, 2013
Last update date Jan 01, 2014
Contact name Yuko Oda
E-mail(s) yuko.oda@ucsf.edu
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL6885
Series (2)
GSE50672 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [10wk keratinocyte]
GSE50673 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury.

Data table header descriptions
ID_REF
VALUE quantile normalized using Genome Studio
p-value

Data table
ID_REF VALUE p-value
ILMN_1248788 106.2 0.20551
ILMN_2707227 115.5 0.02506
ILMN_2896528 2416.4 0
ILMN_2721178 672.6 0
ILMN_1227723 354.6 0
ILMN_3033922 646.7 0
ILMN_3092673 3864.2 0
ILMN_2730714 2205.2 0
ILMN_3162224 763.5 0
ILMN_2816356 107 0.17168
ILMN_2808939 593.3 0
ILMN_2634564 217.7 0
ILMN_1216623 244.3 0
ILMN_1215157 451.1 0
ILMN_1228777 104.1 0.30326
ILMN_2737647 98.3 0.74185
ILMN_1216425 101.6 0.48747
ILMN_2734484 157 0
ILMN_2652961 105.2 0.24687
ILMN_2952292 175.4 0

Total number of rows: 25697

Table truncated, full table size 616 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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