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Sample GSM1226057 Query DataSets for GSM1226057
Status Public on Jan 01, 2014
Title Med1 knockout (KO) keratinocyte rep1
Sample type RNA
Source name Med1 KO_10wk_keratinocyte
Organism Mus musculus
Characteristics strain background: C57BL6
genotype/variation: Med1 KO; MED1 fl/fl K14 driven Cre
age: 10 wk
tissues: skin; epidermis
cell type: keratinocyte
Treatment protocol Keratinocytes were isolated from the skin accoding to the method previously described (Section 3.3 Nowarks JA Fuchs E Mol Biol. 2009 ; 482: 215–232). Briefly, exsided skin was incubated with 0.25% trypsin and epidermis was separated from dermis. Keratinocytes were then isolated from the epidermis.
Growth protocol Coactivator MED1 is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from keratinocytes using RNAeasy RNA purification kit (Qiagen).
Label biotin
Label protocol Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
Hybridization protocol Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
Scan protocol Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
Description KO1
Data processing data were normalized by using quantile method in Genome studio V2011.1
Submission date Sep 06, 2013
Last update date Jan 01, 2014
Contact name Yuko Oda
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platform ID GPL6885
Series (2)
GSE50672 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [10wk keratinocyte]
GSE50673 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury.

Data table header descriptions
VALUE quantile normalized using Genome Studio

Data table
ID_REF VALUE p-value
ILMN_1248788 106.3 0.22306
ILMN_2707227 113.5 0.06015
ILMN_2896528 2886.1 0
ILMN_2721178 626.6 0
ILMN_1227723 455.1 0
ILMN_3033922 742.1 0
ILMN_3092673 4016.9 0
ILMN_2730714 2416.4 0
ILMN_3162224 957.8 0
ILMN_2816356 115.2 0.04135
ILMN_2808939 845.3 0
ILMN_2634564 241.1 0
ILMN_1216623 225.8 0
ILMN_1215157 449.4 0
ILMN_1228777 99.8 0.64662
ILMN_2737647 103.6 0.36591
ILMN_1216425 97.9 0.79323
ILMN_2734484 161 0
ILMN_2652961 95.6 0.92607
ILMN_2952292 187.4 0

Total number of rows: 25697

Table truncated, full table size 619 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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