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Sample GSM1226058 Query DataSets for GSM1226058
Status Public on Jan 01, 2014
Title Med1 knockout (KO) keratinocyte rep2
Sample type RNA
Source name Med1 KO_10wk_keratinocyte
Organism Mus musculus
Characteristics strain background: C57BL6
genotype/variation: Med1 KO; MED1 fl/fl K14 driven Cre
age: 10 wk
tissues: skin; epidermis
cell type: keratinocyte
Treatment protocol Keratinocytes were isolated from the skin accoding to the method previously described (Section 3.3 Nowarks JA Fuchs E Mol Biol. 2009 ; 482: 215–232). Briefly, exsided skin was incubated with 0.25% trypsin and epidermis was separated from dermis. Keratinocytes were then isolated from the epidermis.
Growth protocol Coactivator MED1 is removed from keratin 14 (K14) expressing keratinocytes by using Crelox stragety
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from keratinocytes using RNAeasy RNA purification kit (Qiagen).
Label biotin
Label protocol Standard Illumina hybridization protocol cDNA labeled by Biotin and detected by Cy3-streptoavidin
Hybridization protocol Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
Scan protocol Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
Description KO2
Data processing data were normalized by using quantile method in Genome studio V2011.1
Submission date Sep 06, 2013
Last update date Jan 01, 2014
Contact name Yuko Oda
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platform ID GPL6885
Series (2)
GSE50672 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [10wk keratinocyte]
GSE50673 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury.

Data table header descriptions
VALUE quantile normalized using Genome Studio

Data table
ID_REF VALUE p-value
ILMN_1248788 99.5 0.65915
ILMN_2707227 117.7 0.02632
ILMN_2896528 2836.5 0
ILMN_2721178 663.2 0
ILMN_1227723 366.8 0
ILMN_3033922 792.6 0
ILMN_3092673 4341 0
ILMN_2730714 2730.7 0
ILMN_3162224 936.2 0
ILMN_2816356 112.7 0.0614
ILMN_2808939 809.4 0
ILMN_2634564 230.5 0
ILMN_1216623 233.2 0
ILMN_1215157 448.9 0
ILMN_1228777 93.9 0.94361
ILMN_2737647 102 0.47619
ILMN_1216425 99.6 0.64662
ILMN_2734484 186.8 0
ILMN_2652961 99.7 0.6391
ILMN_2952292 186.9 0

Total number of rows: 25697

Table truncated, full table size 616 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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