|
| Status |
Public on Mar 01, 2007 |
| Title |
Methapyrilene, Low 7 day vs. Methapyrilene, Control 7 day (ExpID 1470B) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Methapyrilene, Low 7 day (Cy3)
|
| Organism |
Rattus norvegicus |
| Characteristics |
Methapyrilene, Low 7 day
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
Methapyrilene, Control 7 day (Cy5)
|
| Organism |
Rattus norvegicus |
| Characteristics |
Methapyrilene, Control 7 day
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
Male Sprague-Dawley rats were treated with 7 daily doses of 10 mg/kg/day methapyrilene or water (vehicle). 24 hours after the last dose, equal amounts of total RNA from 4 methapyrilene-treated rat kidneys was pooled and compared to vehicle RNA (pooled from equal amounts of total RNA from 4 vehicle-treated rat kidneys). RNAs were isolated from kidneys with the RNeasy kit according to manufacturer’s protocols.
|
| Data processing |
Five hundred nanograms (500 ng) of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer’s protocol. For each two color comparison, 750 ng each of Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v. 7.1) using defaults for all parameters.
|
| |
|
| Submission date |
Jul 24, 2006 |
| Last update date |
Dec 22, 2006 |
| Contact name |
James Todd Auman |
| E-mail(s) |
jtauman@email.unc.edu
|
| Phone |
(919) 966-9942
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Institute for Pharmacogenomics and Individualized Therapy
|
| Street address |
3304 Kerr Hall, Campus Box 7360
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7360 |
| Country |
USA |
| |
|
| Platform ID |
GPL890 |
| Series (1) |
| GSE5381 |
Gene expression analysis of liver and kidney following methapyrilene treatment in male Sprague-Dawley rats |
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