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Sample GSM122976 Query DataSets for GSM122976
Status Public on Mar 01, 2007
Title Methapyrilene, High 1 day vs. Methapyrilene, Control 1day (ExpID 1467B)
Sample type RNA
 
Channel 1
Source name Methapyrilene, High 1 day (Cy3)
Organism Rattus norvegicus
Characteristics Methapyrilene, High 1 day
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name Methapyrilene, Control 1day (Cy5)
Organism Rattus norvegicus
Characteristics Methapyrilene, Control 1day
Extracted molecule total RNA
Label Cy5
 
 
Description Male Sprague-Dawley rats were treated with a single dose of 100 mg/kg methapyrilene or water (vehicle). 24 hours after dosing, equal amounts of total RNA from 4 methapyrilene-treated rat kidneys was pooled and compared to vehicle RNA (pooled from equal amounts of total RNA from 4 vehicle-treated rat kidneys). RNAs were isolated from kidneys with the RNeasy kit according to manufacturer’s protocols.
Data processing Five hundred nanograms (500 ng) of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer’s protocol. For each two color comparison, 750 ng each of Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v. 7.1) using defaults for all parameters.
 
Submission date Jul 24, 2006
Last update date Dec 22, 2006
Contact name James Todd Auman
E-mail(s) jtauman@email.unc.edu
Phone (919) 966-9942
Organization name University of North Carolina at Chapel Hill
Department Institute for Pharmacogenomics and Individualized Therapy
Street address 3304 Kerr Hall, Campus Box 7360
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7360
Country USA
 
Platform ID GPL890
Series (1)
GSE5381 Gene expression analysis of liver and kidney following methapyrilene treatment in male Sprague-Dawley rats

Data table header descriptions
ID_REF
rProcessedSignal
gProcessSignal
VALUE Log10(Ratio of Treated:Control)

Data table
ID_REF rProcessedSignal gProcessSignal VALUE
1 1351.265 4477.987 0.52034230048387
2 27.8503 43.20506 0.19070473488625
3 615.5309 646.1179 0.0210619132236592
4 524.2808 638.1266 0.0853428949084507
5 727.3684 753.4899 0.0153230057635102
6 866.3856 1012.776 0.0678021758464656
7 251.0039 3411.671 1.13328667446775
8 1402.876 1549.624 0.0432070484540808
9 111.5304 103.5242 -0.0323513766791491
10 751.9695 759.7059 0.00444527332214076
11 189.1347 167.4803 -0.052807484763713
12 634.4913 765.0806 0.0812815182581879
13 2740.269 3235.419 0.0721373338918471
14 259.4761 3633.308 1.14620485336513
15 9459.783 10139.47 0.0301340804524764
16 799.1742 884.5672 0.0440893765963209
17 1087.994 1224.716 0.051408891263655
18 152.726 182.3098 0.076897037199669
19 1706.265 1602.781 -0.0271722968489256
20 63.59988 83.51693 0.118318225490559

Total number of rows: 21575

Table truncated, full table size 900 Kbytes.




Supplementary file Size Download File type/resource
GSM122976.tif.gz 24.8 Mb (ftp)(http) TIFF
GSM122976.txt.gz 5.0 Mb (ftp)(http) TXT

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