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Sample GSM122990 Query DataSets for GSM122990
Status Public on Mar 01, 2007
Title MP G7 control 7 days vs. MP G8 high 7 days (ExpID 1996B)
Sample type RNA
 
Channel 1
Source name MP G7 control 7 days (Cy3)
Organism Rattus norvegicus
Characteristics MP G7 control 7 days
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name MP G8 high 7 days (Cy5)
Organism Rattus norvegicus
Characteristics MP G8 high 7 days
Extracted molecule total RNA
Label Cy5
 
 
Description Male Sprague-Dawley rats were treated with 7 daily doses of 100 mg/kg/day methapyrilene or water (vehicle). 24 hours after the last dose, equal amounts of total RNA from 4 methapyrilene-treated rat livers was pooled and compared to vehicle RNA (pooled from equal amounts of total RNA from 4 vehicle-treated rat livers). RNAs were isolated from livers with the RNeasy kit according to manufacturer’s protocols.
Data processing Five hundred nanograms (500 ng) of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer’s protocol. For each two color comparison, 750 ng each of Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v. 7.1) using defaults for all parameters.
 
Submission date Jul 24, 2006
Last update date Dec 22, 2006
Contact name James Todd Auman
E-mail(s) jtauman@email.unc.edu
Phone (919) 966-9942
Organization name University of North Carolina at Chapel Hill
Department Institute for Pharmacogenomics and Individualized Therapy
Street address 3304 Kerr Hall, Campus Box 7360
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7360
Country USA
 
Platform ID GPL890
Series (1)
GSE5381 Gene expression analysis of liver and kidney following methapyrilene treatment in male Sprague-Dawley rats

Data table header descriptions
ID_REF
rProcessedSignal
gProcessSignal
VALUE Log10(Ratio of Treated:Control)

Data table
ID_REF rProcessedSignal gProcessSignal VALUE
1 180.8511 4755.01 -1.41983027997777
2 45.32215 77.74771 -0.234377102254859
3 81.74867 44.23493 0.266715351334332
4 115.7601 149.7647 -0.111850567602625
5 163.5017 85.06643 0.283764065509594
6 217.9491 248.8507 -0.0575837867426297
7 68.96673 3717.833 -1.73165024319042
8 275.6733 79.04294 0.542531619630813
9 49.58117 30.46707 0.211486080500982
10 781.46 366.3534 0.329006526590484
11 52.55269 32.62772 0.207008224361158
12 164.6647 102.9356 0.204034906936844
13 828.5052 864.2423 -0.0183402803142467
14 46.52757 3992.13 -1.93349430405674
15 12660 8247.959 0.186087212246566
16 244.8539 268.7392 -0.0404239942012813
17 152.4833 126.383 0.0815336221139967
18 61.81032 175.7841 -0.453918597771411
19 296.0215 321.2273 -0.0354891924130988
20 56.63521 171.6504 -0.48155830461938

Total number of rows: 21575

Table truncated, full table size 889 Kbytes.




Supplementary file Size Download File type/resource
GSM122990.tif.gz 24.7 Mb (ftp)(http) TIFF
GSM122990.txt.gz 5.0 Mb (ftp)(http) TXT

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