|
| Status |
Public on May 08, 2014 |
| Title |
Middle aged 2 |
| Sample type |
RNA |
| |
|
| Source name |
Skeletal muscle
|
| Organism |
Mus musculus |
| Characteristics |
cell type: skeletal muscle precursor cells
cell surface markers: CD45-Ter119-Sca-1-CD29+Cxcr4+
developmental stage: Middle aged (12 months)
genetic background: C57BL/6
|
| Treatment protocol |
Myofiber-associated cells were isolated from mouse muscle by two-step enzymatic digestion and mechanical dissociation. Individual cell subsets were isolated by combinatorial cell surface marker staining and fluorescence-activated cell sorting.
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from total RNA using 3'IVT ExpressKit from Affymetrix.
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized to the GeneChip® Mouse Genome 430 2.0 Array, washed and stained according to standard Affymetrix protocol (Affymetrix Hybridization, Wash and Stain kit).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from middle aged mouse skeletal muscle precursor cells
|
| Data processing |
Data were normalized using RMA (Robust Multi-array average).
Data were analyzed using GenePattern.
|
| |
|
| Submission date |
Sep 12, 2013 |
| Last update date |
May 08, 2014 |
| Contact name |
Amy J Wagers |
| E-mail(s) |
amy_wagers@harvard.edu
|
| Phone |
+1-617-309-2590
|
| Organization name |
Joslin diabetes center and Harvard University
|
| Department |
Stem Cell and Regenerative Biology
|
| Lab |
Stem Cell and Regenerative Biology
|
| Street address |
One Joslin place
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE50821 |
Restoring Systemic GDF11 Levels Reverses Age-Related Dysfunction in Mouse Skeletal Muscle |
|
