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Sample GSM1230359 Query DataSets for GSM1230359
Status Public on May 08, 2014
Title Middle aged 4
Sample type RNA
 
Source name Skeletal muscle
Organism Mus musculus
Characteristics cell type: skeletal muscle precursor cells
cell surface markers: CD45-Ter119-Sca-1-CD29+Cxcr4+
developmental stage: Middle aged (12 months)
genetic background: C57BL/6
Treatment protocol Myofiber-associated cells were isolated from mouse muscle by two-step enzymatic digestion and mechanical dissociation. Individual cell subsets were isolated by combinatorial cell surface marker staining and fluorescence-activated cell sorting.
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA was prepared from total RNA using 3'IVT ExpressKit from Affymetrix.
 
Hybridization protocol Biotinylated cRNA was hybridized to the GeneChip® Mouse Genome 430 2.0 Array, washed and stained according to standard Affymetrix protocol (Affymetrix Hybridization, Wash and Stain kit).
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from middle aged mouse skeletal muscle precursor cells
Data processing Data were normalized using RMA (Robust Multi-array average).
Data were analyzed using GenePattern.
 
Submission date Sep 12, 2013
Last update date May 08, 2014
Contact name Amy J Wagers
E-mail(s) amy_wagers@harvard.edu
Phone +1-617-309-2590
Organization name Joslin diabetes center and Harvard University
Department Stem Cell and Regenerative Biology
Lab Stem Cell and Regenerative Biology
Street address One Joslin place
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE50821 Restoring Systemic GDF11 Levels Reverses Age-Related Dysfunction in Mouse Skeletal Muscle

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1415670_at 97.44906775
1415671_at 317.228648
1415672_at 188.4386496
1415673_at 51.03804922
1415674_a_at 178.9982158
1415675_at 203.7993435
1415676_a_at 726.6974301
1415677_at 24.07613471
1415678_at 1272.561758
1415679_at 742.2498648
1415680_at 144.1998641
1415681_at 157.0656157
1415682_at 20.50603418
1415683_at 931.5389847
1415684_at 26.47762555
1415685_at 29.76899227
1415686_at 203.6974301
1415687_a_at 897.1635156
1415688_at 289.2854827
1415689_s_at 86.13754076

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1230359_MIDDLE_AGED_4.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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