|
| Status |
Public on Sep 17, 2013 |
| Title |
2556 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
mouse heart graft tissue without 18h cold ischemia before heart transplantation
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
tissue: heart tissue without 18h cold ischemia reperfusion
time point: 24h after transplantation
|
| Treatment protocol |
Donor hearts were preserved with University of Wisconsin solution for 18h at 4 C degree before being implanted into recipients.
|
| Growth protocol |
heart grafts were harvested from recipients 24h after heart transplantation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using miRNEasy mini kit from Qiagen according to manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
1.0 ug of total RNA was labeled using the Flash Tag Biotin HSR kit form Genisphere
|
| |
|
| Hybridization protocol |
1.0 ug of labeled RNA was hybridized for 16 hr at 48C on GeneChip miRNA 3.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
M0047.CEL
|
| Data processing |
The data were analyzed with Partek Genomcis Suite(v.6.6) using the RMA normalization method
|
| |
|
| Submission date |
Sep 16, 2013 |
| Last update date |
Sep 17, 2013 |
| Contact name |
Xiufen Zheng |
| E-mail(s) |
xzheng26@uwo.ca
|
| Organization name |
university of Western Ontario
|
| Department |
Pathology
|
| Street address |
339 windermere Road,LHSC-UC, B4-235
|
| City |
Lodnon |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 5A5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16384 |
| Series (2) |
| GSE50885 |
microRNA expression in heart tissue |
| GSE50886 |
microRNA and gene expression profile in ischemia reperfusion injury in heart transplantation |
|
