|
| Status |
Public on Nov 19, 2013 |
| Title |
WT at stage 2 iNKT, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Sorted Tet+CD24-CD44+NK1.1- iNKT cells from WT thymus
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
cell type: Sorted Tet+CD24-CD44+NK1.1- iNKT cells from WT thymus
tissue: Thymus
strain: C57BL/6
age: 6 weeks old
|
| Treatment protocol |
Thymocyte single cell suspensions were surface stained and FACS sorted into RLT-containing (Qiagen) eppendorf tubes, before freezing in dry ice. Cells were stored in -80oC until RNA extraction was performed.
|
| Growth protocol |
Mice were housed at the Univeristy of Chicago Animal Resource Center, following the guidelines of The University of Chicago Institutional Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the Affymetrix two-cycle cDNA sunthesis.
|
| |
|
| Hybridization protocol |
10 ug of fragmented cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array 430.2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
|
| Description |
Gene expression data from mouse WT Stage 2 iNKT cells
|
| Data processing |
Expression values were calculated by RMA express (http://rmaexpress.bmbolstad.com/) using background adjustment and quantile normalization.
|
| |
|
| Submission date |
Sep 17, 2013 |
| Last update date |
Nov 19, 2013 |
| Contact name |
Barbara L Kee |
| Organization name |
The University of Chicago
|
| Department |
Pathology
|
| Street address |
924 E57th st
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE50933 |
Essential functions for ID proteins at multiple checkpoints in natural killer T cell development |
|
