|
| Status |
Public on Jun 30, 2014 |
| Title |
PBMC_placebo_visit2_rep20 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Peripheral blood mononuclear cells, placebo
|
| Organism |
Homo sapiens |
| Characteristics |
agent: placebo
timepoint: Day 1
cell type: Peripheral blood mononuclear cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Stratagene Universal Human Reference RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
|
| |
|
| |
| Hybridization protocol |
The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
|
| Scan protocol |
Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
|
| Description |
20PLCv2
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 17, 2013 |
| Last update date |
Jun 30, 2014 |
| Contact name |
Phillip Monk |
| Organization name |
Synairgen Research Ltd
|
| Department |
Southampton General Hospital
|
| Street address |
Tremona Road
|
| City |
Southampton |
| ZIP/Postal code |
SO16 6YD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE50943 |
The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection |
|
