NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1232912 Query DataSets for GSM1232912
Status Public on Jun 30, 2014
Title PBMC_SNG001_visit2_rep21
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, SNG001
Organism Homo sapiens
Characteristics agent: SNG001
timepoint: Day 1
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 21SNGv2
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.5165923
DarkCorner 0.11442705
A_23_P117082 -0.03858369
A_33_P3246448 -4.3804126
A_33_P3318220 0.10596316
A_33_P3236322 0.10917515
A_33_P3319925 0.4102435
A_21_P0000509 -5.3205423
A_21_P0000744 -1.3114424
A_24_P215804 1.1123443
A_23_P110167 -0.8969002
A_33_P3211513 0.7948712
A_23_P103349 -0.07891127
A_32_P61480 0.12838364
A_33_P3788124 0.12984568
A_33_P3414202 1.5354031
A_33_P3316686 2.0173588
A_33_P3300975 -0.9063643
A_33_P3263061 0.021983199
A_33_P3261373 0.13486966

Total number of rows: 50739

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1232912_21SNGv2.txt.gz 21.4 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap