|
| Status |
Public on Nov 04, 2014 |
| Title |
agLDL WT_2.2 |
| Sample type |
RNA |
| |
|
| Source name |
BMDM, Ndfst1 wild type, 50 µg/ml agLDL
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL6
age: 12 week old
cell type: bone marrow derived macrophages
phenotype: Ndfst1 wild type
|
| Treatment protocol |
BMDM were treated with or without 50 µg/ml of aggregated LDL for 24 hours in DMEM/F12 medium supplemented with 10% lipoprotein-eficient fetal calf serum, 100 units/ml penicillin, 100 μg/ml streptomycin and 20 ng/ml recombinant mouse Macrophage Colony Stimulatinf Factor 1
|
| Growth protocol |
BMDM were grown in DMEM/F12 medium supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 μg/ml streptomycin and 20 ng/ml recombinant mouse Macrophage Colony Stimulatinf Factor 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
standard Illumina
|
| |
|
| Hybridization protocol |
standard Illumina
|
| Scan protocol |
standard Illumina
|
| Description |
Ndst1f/fLysMCre- mouse 2
Replicate 2
6668263002_F
|
| Data processing |
Illumina GenomeStudio: cubic spline normalization
|
| |
|
| Submission date |
Sep 19, 2013 |
| Last update date |
Nov 04, 2014 |
| Contact name |
Philip Leo Stefan Maria Gordts |
| E-mail(s) |
pgordts@ucsd.edu
|
| Organization name |
UCSD
|
| Department |
CMM
|
| Lab |
Esko Lab
|
| Street address |
9500 Gilman Dr #0687
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE51009 |
Gene expression in macrophages derived form wild-type and Ndfst1-deficient mice before and after foam cell conversion |
|
