|
| Status |
Public on Oct 03, 2006 |
| Title |
hES cells differentiated to an fibroblast-like cell type |
| Sample type |
RNA |
| |
|
| Source name |
HEF cells derived from H9 hESCs
|
| Organism |
Homo sapiens |
| Characteristics |
Cells displayed fibroblast-like morphology and are capable of supporting the undifferentiated growth of parantal hES cells.
|
| Treatment protocol |
Fibroblast-like differentiated cells were derived from differentiated H9 hES cells grown in MEF-conditioned medium prepared without FGF2 supplementation in the conditioning step. Fibroblast-like cells were enriched by mechanical removal of remaining undifferentiated colonies.
|
| Growth protocol |
The differentiated cells were further selected by trypsinisation and expanded in serum-containing medium before RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit with on-column DNA digestion following the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Linear amplification kit Ambion #IL1791 following the manufacturer's instructions. Input amount: 300ng of total RNA; IVT: 6h
|
| |
|
| Hybridization protocol |
Employing materials and protocols by Illumina Inc.; hybridisation: 17h at 55 degC
|
| Scan protocol |
Using an Illumina scanner; gain factor: 2.5
|
| Description |
The differentiated human embryonic fibroblast-like (HEF) cells did not express markers of the undifferentiated state but could instead be used for conditioning hES medium.
|
| Data processing |
In BeadStudio 1.0 raw data were background subtracted and normalised using the "rank invariant" algorithm. Value = "null" denotes expression below background (p < 0.01).
|
| |
|
| Submission date |
Jul 31, 2006 |
| Last update date |
Oct 03, 2006 |
| Contact name |
Boris Greber |
| E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2700 |
| Series (1) |
| GSE5416 |
Comparative transcriptome analysis of four cell lines |
|
