|
| Status |
Public on Jun 01, 2007 |
| Title |
SOX2 knock-down in NCCIT hEC cells |
| Sample type |
RNA |
| |
|
| Source name |
cell line NCCIT
|
| Organism |
Homo sapiens |
| Characteristics |
human embryonic carcinoma cell line NCCIT
|
| Treatment protocol |
Cells were seeded at 70,000/sqcm and treated with esiRNA against SOX2 message complexed with Hyperfect transfection reagent (Qiagen), following the vendor's recommendations. Medium was changed the following day and cells harvested at day 2.5
|
| Growth protocol |
high-glucose DMEM supplemented with 10% FCS, 2mM L-glutamine, penicillin-streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit with on-column DNA digestion following the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Linear amplification kit Ambion #IL1791 following the manufacturer's instructions. Input amount: 300ng of total RNA; IVT: 6h
|
| |
|
| Hybridization protocol |
Employing materials and protocols by Illumina Inc.; hybridisation: 17h at 55 degC
|
| Scan protocol |
Using an Illumina scanner; gain factor: 2.5
|
| Description |
The control for this knock-down experiment is named NCCIT hEC cells mock-treated. Values are means of three biological replicates.
|
| Data processing |
In BeadStudio 1.0 raw data were background subtracted and normalised using the "rank invariant" algorithm. Value = "null" denotes expression below background (p < 0.01).
|
| |
|
| Submission date |
Aug 01, 2006 |
| Last update date |
Jun 01, 2007 |
| Contact name |
Boris Greber |
| E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2507 |
| Series (1) |
| GSE5422 |
Knock-down of three core transcription factors in hEC cells |
|
