|
| Status |
Public on Oct 31, 2008 |
| Title |
Foxa2-mutant-2-E7.5 (U74) |
| Sample type |
RNA |
| |
|
| Source name |
E7.5 EMBRYO EPIBLAST ONLY
|
| Organism |
Mus musculus |
| Characteristics |
Embryos derived by tetraploid complementation technique using Foxa2 homozygous null ES cells.
|
| Treatment protocol |
EXTRA-EMBRYONIC REGION OF EMBRYO REMOVED PRIOR TO EXTRACTION
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL REAGENT
|
| Label |
biotin
|
| Label protocol |
AFFYMETRIX GeneChip® T7-Oligo(dT) Promoter Primer Kit
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
E7.5 POOLED EMBRYOS: n=48
Stages between mid-streak and headfold according to Downs & Davies
|
| Data processing |
Affymetrix MAS5.0, target normalized to 1000
|
| |
|
| Submission date |
Aug 01, 2006 |
| Last update date |
Oct 31, 2008 |
| Contact name |
Owen James Tamplin |
| Organization name |
University of Wisconsin-Madison
|
| Department |
Cell and Regenerative Biology
|
| Lab |
Tamplin
|
| Street address |
1111 Highland Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5424 |
Microarray analysis of Foxa2 mutant mouse embryos |
|
