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Sample GSM1243877 Query DataSets for GSM1243877
Status Public on Jan 01, 2014
Title 1351
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IV
classification: chemotherapy sensitive
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 6705.558997
1053_at 422.0887818
117_at 336.9064921
121_at 7076.127861
1255_g_at 12.89867804
1294_at 1848.139167
1316_at 174.2097073
1320_at 102.0248183
1405_i_at 6122.341601
1431_at 430.5490061
1438_at 97.30230604
1487_at 1021.781088
1494_f_at 57.57139394
1552256_a_at 568.8888618
1552257_a_at 2379.12999
1552258_at 153.8033565
1552261_at 991.0123798
1552263_at 250.3380048
1552264_a_at 154.9460192
1552266_at 19.38705519

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243877_U133Plus2_S1351.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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