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Sample GSM1243878 Query DataSets for GSM1243878
Status Public on Jan 01, 2014
Title 1413
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IV
classification: chemotherapy resistant
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 13794.48461
1053_at 480.8816492
117_at 132.6881807
121_at 1673.255375
1255_g_at 45.16790876
1294_at 2213.35945
1316_at 185.1541532
1320_at 32.93912739
1405_i_at 513.5589062
1431_at 498.9720083
1438_at 42.37490801
1487_at 1057.847405
1494_f_at 206.3957939
1552256_a_at 1390.861909
1552257_a_at 1810.117616
1552258_at 84.15850728
1552261_at 135.810961
1552263_at 141.9292485
1552264_a_at 72.11522469
1552266_at 83.96610667

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243878_U133Plus2_R1413.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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