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Sample GSM1243880 Query DataSets for GSM1243880
Status Public on Jan 01, 2014
Title 1100
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIIc
classification: chemotherapy resistant
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 4405.443255
1053_at 1346.884042
117_at 108.5325306
121_at 446.8982477
1255_g_at 19.95911
1294_at 497.910458
1316_at 283.8568247
1320_at 69.90584722
1405_i_at 50.79492088
1431_at 29.12734894
1438_at 1956.135935
1487_at 2719.232719
1494_f_at 100.0492591
1552256_a_at 2413.267137
1552257_a_at 2424.578777
1552258_at 119.7043469
1552261_at 51.4898419
1552263_at 469.1813812
1552264_a_at 406.445088
1552266_at 192.5885813

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243880_U133Plus2_R1100.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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