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Sample GSM1243883 Query DataSets for GSM1243883
Status Public on Jan 01, 2014
Title 1157
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIIc
classification: chemotherapy sensitive
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 4574.101155
1053_at 590.5508142
117_at 519.6248114
121_at 6211.525281
1255_g_at 38.50442661
1294_at 272.4442377
1316_at 207.5920545
1320_at 84.03072784
1405_i_at 89.74226358
1431_at 134.2460028
1438_at 179.7548237
1487_at 863.0011214
1494_f_at 82.0128741
1552256_a_at 552.8610491
1552257_a_at 694.9078493
1552258_at 38.54116925
1552261_at 556.4258054
1552263_at 127.9918337
1552264_a_at 86.2002518
1552266_at 30.88229523

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM1243883_U133Plus2_S1157.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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