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Sample GSM1243890 Query DataSets for GSM1243890
Status Public on Jan 01, 2014
Title 1381
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIIa
classification: chemotherapy sensitive
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 12565.42325
1053_at 924.0189687
117_at 131.7184528
121_at 6681.265773
1255_g_at 43.12248992
1294_at 1681.570182
1316_at 183.5915103
1320_at 44.37753671
1405_i_at 82.17796671
1431_at 252.0296848
1438_at 318.3139519
1487_at 766.557641
1494_f_at 66.47748648
1552256_a_at 733.441669
1552257_a_at 1646.39781
1552258_at 103.1051322
1552261_at 256.7089297
1552263_at 90.87718484
1552264_a_at 90.55685882
1552266_at 290.8107636

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243890_U133Plus2_S1381.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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