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Sample GSM1243893 Query DataSets for GSM1243893
Status Public on Jan 01, 2014
Title 1605
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIb
classification: chemotherapy resistant
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 10523.2603
1053_at 605.1991407
117_at 145.5082795
121_at 15736.86892
1255_g_at 26.666315
1294_at 918.2423439
1316_at 195.0420463
1320_at 53.52134953
1405_i_at 384.0513689
1431_at 157.1120861
1438_at 201.5893987
1487_at 450.4340842
1494_f_at 125.4295941
1552256_a_at 1296.663844
1552257_a_at 1252.074864
1552258_at 133.2616761
1552261_at 320.1090257
1552263_at 178.6179044
1552264_a_at 139.7965952
1552266_at 31.12040837

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243893_U133Plus2_R1605.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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