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Sample GSM1243894 Query DataSets for GSM1243894
Status Public on Jan 01, 2014
Title 1776
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIIc
classification: chemotherapy resistant
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 11365.65592
1053_at 470.2304863
117_at 113.0590652
121_at 11280.29264
1255_g_at 5.116733801
1294_at 676.56689
1316_at 153.2481987
1320_at 99.9003681
1405_i_at 5.325726559
1431_at 741.8928805
1438_at 180.6244661
1487_at 675.6177596
1494_f_at 116.7945245
1552256_a_at 654.14823
1552257_a_at 477.0860853
1552258_at 87.30267755
1552261_at 1279.292295
1552263_at 83.75207701
1552264_a_at 45.03103526
1552266_at 213.8694699

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243894_U133Plus2_R1776.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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