|
| Status |
Public on Jan 01, 2014 |
| Title |
1299 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Ovary
Stage: IIIb
classification: chemotherapy resistant
|
| Growth protocol |
Fresh frozen tumour samples stored at -80 C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
|
| Scan protocol |
Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
|
| Data processing |
Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
|
| |
|
| Submission date |
Oct 17, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Madhuri Koti |
| Organization name |
Queen's University
|
| Department |
Biomedical and Molecular Sciences
|
| Street address |
Botterell Hall, Stuart Street
|
| City |
Kingston |
| State/province |
Ontario |
| ZIP/Postal code |
K7P3E3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE51373 |
Gene expression data from high grade serous ovarian cancer |
|
