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Sample GSM1243897 Query DataSets for GSM1243897
Status Public on Jan 01, 2014
Title 1359
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIIc
classification: chemotherapy resistant
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 9277.393175
1053_at 428.3684159
117_at 117.7840223
121_at 6365.814213
1255_g_at 57.21106194
1294_at 697.443596
1316_at 192.3901724
1320_at 92.44233939
1405_i_at 1099.251701
1431_at 175.5008486
1438_at 215.8385012
1487_at 534.2720929
1494_f_at 102.4831893
1552256_a_at 431.2959777
1552257_a_at 927.5298959
1552258_at 83.79351044
1552261_at 2094.807163
1552263_at 508.4639388
1552264_a_at 399.5154282
1552266_at 43.39035983

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243897_U133Plus2_R1359.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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