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Sample GSM1243898 Query DataSets for GSM1243898
Status Public on Jan 01, 2014
Title 1355
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIa
classification: chemotherapy sensitive
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 5069.514813
1053_at 408.8814775
117_at 221.5043444
121_at 7076.820441
1255_g_at 4.408704196
1294_at 2883.78826
1316_at 48.19760744
1320_at 112.3814566
1405_i_at 2254.039171
1431_at 82.81057721
1438_at 187.196301
1487_at 1326.930256
1494_f_at 111.6995003
1552256_a_at 900.8121541
1552257_a_at 888.7716744
1552258_at 164.9069859
1552261_at 1295.748772
1552263_at 555.1321525
1552264_a_at 453.8770096
1552266_at 64.36416111

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1243898_U133Plus2_S1355.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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