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Sample GSM1243903 Query DataSets for GSM1243903
Status Public on Jan 01, 2014
Title B01360
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics tissue: Ovary
Stage: IIIc
classification: chemotherapy sensitive
Growth protocol Fresh frozen tumour samples stored at -80 C.
Extracted molecule total RNA
Extraction protocol RNA was extracted as usiing Qiagen Rneasy mini kit as per the manufacturer's protocol
Label Biotin
Label protocol GeneChip Two-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA)
 
Hybridization protocol 500 nanograms of total RNA was used for cDNA synthesis using GeneChip 3′ IVT Express Kit (Affymetrix Inc, Santa Clara, CA USA.)
Scan protocol Scanning and probe quantification of the gene chips was performed on an AffymetrixGeneChip Scanner 3000 as per the manufacturer's instructions.
Data processing Normalization of the microarray data was conducted using MAS5 algorithm from the affy Bioconductor package.
 
Submission date Oct 17, 2013
Last update date Jan 01, 2014
Contact name Madhuri Koti
Organization name Queen's University
Department Biomedical and Molecular Sciences
Street address Botterell Hall, Stuart Street
City Kingston
State/province Ontario
ZIP/Postal code K7P3E3
Country Canada
 
Platform ID GPL570
Series (1)
GSE51373 Gene expression data from high grade serous ovarian cancer

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 7047.526184
1053_at 503.4770357
117_at 230.610954
121_at 11701.89073
1255_g_at 50.29396431
1294_at 832.7263343
1316_at 88.09448156
1320_at 76.95741
1405_i_at 217.1149222
1431_at 61.22835695
1438_at 153.3783474
1487_at 1066.181999
1494_f_at 160.3863246
1552256_a_at 447.7509627
1552257_a_at 1940.716717
1552258_at 158.2004769
1552261_at 1153.110426
1552263_at 264.0872538
1552264_a_at 191.5072419
1552266_at 137.179888

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM1243903_U133Plus2_SB01440.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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