|
| Status |
Public on Oct 19, 2013 |
| Title |
07 Heterozygous Deletion (miR-1) |
| Sample type |
RNA |
| |
|
| Source name |
Heterozygous Deletion (miR-1)
|
| Organism |
Mus musculus |
| Characteristics |
strain: Mixed 129 x C57BL/6
tissue: Neonatal hearts (P2)
genotype/variation: miR-1 DOUBLE HETEROZYGOUS DELETION
|
| Treatment protocol |
Neonatal hearts (P2) from wildtype, miR-1-1 null and miR-1-2 +/-: miR-1-1 +/- double heterozygote animals were isolated
|
| Growth protocol |
Samples are from neonatal hearts (P2) from wildtype, miR-1-1 null and miR-1-2 +/-: miR-1-1 +/- double heterozygote mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol (Invitrogen), following the manufacturer’s suggested protocol.
amplication protocol: NuGen Ovation pico WTA system v2
|
| Label |
biotin
|
| Label protocol |
Genomic DNA was removed using a gDNA eliminator column (Qiagen)
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix mouse genechip ST 2.0 arrays: GeneChip Expression Analysis Technical Manual 702232 Rev. 3
|
| Scan protocol |
Affymetrix GCS3000
|
| Data processing |
CEL files were generated using AGCC 4.0.0.1567G. The values in the CEL files were then normalized with the RMA normalization method, using the Affymetrix Power Tools suite, version 1.12.0.
|
| |
|
| Submission date |
Oct 18, 2013 |
| Last update date |
Oct 19, 2013 |
| Contact name |
Gladstone Bioinformatics |
| Organization name |
Gladstone Institutes
|
| Department |
Data Science and Biotechnology
|
| Lab |
Bioinformatics Core
|
| Street address |
1650 Owens St
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE51394 |
Wildtype, miR-1-1 KO, miR-1 Double het P2 mixed strain heart analysis (MoGene 2.0 ST Arrays). |
|
