NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM124487 Query DataSets for GSM124487
Status Public on Aug 02, 2006
Title embryo at 4-6hour, fraction 8, biological rep1
Sample type RNA
 
Source name Drosophila embryos
Organism Drosophila melanogaster
Characteristics Canon S embryos
Treatment protocol embryos were collected in 2-hour intervals and aged to generate animals 0-2, 4-6 and 8-10 hour old.
Extracted molecule total RNA
Extraction protocol the dechorionated embryos were first incubated with 0.1mg/ml cycloheximide in PBS for 10 minutes on ice, then homogenized with a motorized plastic pellet pestle in a lysis buffer (20mM Tris-HCl, pH7.4, 140mM Kcl, 5mM MgCl2, 0.5mM DTT, 1% Triton X-100, 0.1mg/ml cycloheximide, 1mg/ml heparin, 50unit/ml RNasin) and incubated for 10 minutes on ice. The nuclei and debris were removed by centrifugation at 12,000x g for 10min at 4oC, and supernatants was loaded onto 20-50% sucrose gradients with the same extraction buffer without Triton x-100.
Label biotin
 
Hybridization protocol Following fragmentation, at least 10 microg of cRNA were hybridized on GeneChip Drosophila Genome 2.0 Array.
Description Gene expression data from embryos during first 10 hours after egg laying to see the global and transcript-specific translation profile
Data processing Gene expression measures were computed using the Robust Multichip Average (RMA) method and implemented in the Bioconductor R package.
 
Submission date Aug 01, 2006
Last update date Aug 28, 2018
Contact name Soyeon Ahn
E-mail(s) ahn@stat.berkeley.edu
Organization name UC Berkeley
Street address 367 Evans Hall #3860
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platform ID GPL1322
Series (1)
GSE5430 Expression data from early Drosophila embryo
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE intensity after RMA

Data table
ID_REF VALUE
1616608_a_at 8.880735379
1622892_s_at 9.606066113
1622893_at 2.970209146
1622894_at 4.444865147
1622895_at 8.503925883
1622896_at 4.218212983
1622897_at 6.020330791
1622898_a_at 11.10735309
1622899_at 5.457121104
1622900_at 3.337934924
1622901_at 3.834538876
1622902_at 4.3551008
1622903_s_at 8.652917337
1622904_at 4.812582166
1622905_at 3.685483102
1622906_at 5.871549294
1622907_at 9.025981626
1622908_a_at 5.561701524
1622909_at 6.165744381
1622910_at 3.588192534

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM124487.CEL.gz 3.2 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap