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Sample GSM124498 Query DataSets for GSM124498
Status Public on Aug 02, 2006
Title embryo at 4-6hour, fraction 11, biological rep2
Sample type RNA
 
Source name Drosophila embryos
Organism Drosophila melanogaster
Characteristics Canon S embryos
Treatment protocol embryos were collected in 2-hour intervals and aged to generate animals 0-2, 4-6 and 8-10 hour old.
Extracted molecule total RNA
Extraction protocol the dechorionated embryos were first incubated with 0.1mg/ml cycloheximide in PBS for 10 minutes on ice, then homogenized with a motorized plastic pellet pestle in a lysis buffer (20mM Tris-HCl, pH7.4, 140mM Kcl, 5mM MgCl2, 0.5mM DTT, 1% Triton X-100, 0.1mg/ml cycloheximide, 1mg/ml heparin, 50unit/ml RNasin) and incubated for 10 minutes on ice. The nuclei and debris were removed by centrifugation at 12,000x g for 10min at 4oC, and supernatants was loaded onto 20-50% sucrose gradients with the same extraction buffer without Triton x-100.
Label biotin
 
Hybridization protocol Following fragmentation, at least 10 microg of cRNA were hybridized on GeneChip Drosophila Genome 2.0 Array.
Description Gene expression data from embryos during first 10 hours after egg laying to see the global and transcript-specific translation profile
Data processing Gene expression measures were computed using the Robust Multichip Average (RMA) method and implemented in the Bioconductor R package.
 
Submission date Aug 01, 2006
Last update date Aug 28, 2018
Contact name Soyeon Ahn
E-mail(s) ahn@stat.berkeley.edu
Organization name UC Berkeley
Street address 367 Evans Hall #3860
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platform ID GPL1322
Series (1)
GSE5430 Expression data from early Drosophila embryo
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE intensity after RMA

Data table
ID_REF VALUE
1616608_a_at 9.542173394
1622892_s_at 8.922083496
1622893_at 3.144220341
1622894_at 4.646499079
1622895_at 10.59845766
1622896_at 4.213189385
1622897_at 6.713565197
1622898_a_at 9.034881725
1622899_at 4.311109227
1622900_at 3.297108065
1622901_at 4.007370443
1622902_at 4.731368791
1622903_s_at 7.326014453
1622904_at 5.214775551
1622905_at 3.785819657
1622906_at 5.629222493
1622907_at 7.36144569
1622908_a_at 4.718457173
1622909_at 7.59045123
1622910_at 3.84418511

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM124498.CEL.gz 3.2 Mb (ftp)(http) CEL

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