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Sample GSM1245165 Query DataSets for GSM1245165
Status Public on Jan 02, 2014
Title leukemiaRNA_TCL1_1
Sample type RNA
 
Source name TCL1 transgenic mouse leukemia RNA
Organism Mus musculus
Characteristics transgenic mice: TCL1 mouse
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from leukemia cells using TRIzol® reagent (Life Technologies,Carlsbad, CA) according to the manufacturer’s protocol followed by Turbo DNase treatment (Life Technologies, Carlsbad, CA) to remove trace amounts of genomic DNA.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the Ambion WT Expression Kit (Applied Biosystems) and then fragmented and labeled with the GeneChip WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the UCSD GeneChip Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from BioConductor Oligo package
Data processing Raw data were processed with the BioConductor Oligo package for background correction and normalization. Data analysis and statistical evaluations were performed in R (version 2.15.1, R Core Team (2012). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.r-project.org/).
 
Submission date Oct 18, 2013
Last update date Jan 02, 2014
Contact name Emanuela Maria Ghia
E-mail(s) eghia@health.ucsd.edu
Organization name University of California San Diego
Department Center For Novel Therapeutics
Street address 9310 Athena Circle
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL6193
Series (1)
GSE51420 ROR1 Can Interact With TCL1 And Enhance Leukemogenesis in Eµ-TCL1 Transgenic Mice

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
4304920 0.994882774372222
4304921 1.24692854847436
4304922 7.21762587510416
4304923 5.17235037422342
4304925 1.38896459880109
4304927 3.03960510760174
4304928 7.25727556760058
4304929 9.07715999435335
4304930 4.25905521504672
4304931 1.52051795998099
4304932 3.40463995719883
4304933 5.52346904494505
4304934 2.11949606520655
4304935 1.77233412279059
4304937 2.50692965053053
4304938 7.08052445968496
4304939 0.934990219867746
4304940 3.27883805758127
4304941 2.77647001626379
4304942 6.99584646393876

Total number of rows: 1236087

Table truncated, full table size 30084 Kbytes.




Supplementary file Size Download File type/resource
GSM1245165_13277_300-+.CEL.gz 19.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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