|
| Status |
Public on Jan 02, 2014 |
| Title |
leukemiaRNA_TCL1_1 |
| Sample type |
RNA |
| |
|
| Source name |
TCL1 transgenic mouse leukemia RNA
|
| Organism |
Mus musculus |
| Characteristics |
transgenic mice: TCL1 mouse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from leukemia cells using TRIzol® reagent (Life Technologies,Carlsbad, CA) according to the manufacturer’s protocol followed by Turbo DNase treatment (Life Technologies, Carlsbad, CA) to remove trace amounts of genomic DNA.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the Ambion WT Expression Kit (Applied Biosystems) and then fragmented and labeled with the GeneChip WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the UCSD GeneChip Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from BioConductor Oligo package
|
| Data processing |
Raw data were processed with the BioConductor Oligo package for background correction and normalization. Data analysis and statistical evaluations were performed in R (version 2.15.1, R Core Team (2012). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org/).
|
| |
|
| Submission date |
Oct 18, 2013 |
| Last update date |
Jan 02, 2014 |
| Contact name |
Emanuela Maria Ghia |
| E-mail(s) |
eghia@health.ucsd.edu
|
| Organization name |
University of California San Diego
|
| Department |
Center For Novel Therapeutics
|
| Street address |
9310 Athena Circle
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE51420 |
ROR1 Can Interact With TCL1 And Enhance Leukemogenesis in Eµ-TCL1 Transgenic Mice |
|
