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Sample GSM1245166 Query DataSets for GSM1245166
Status Public on Jan 02, 2014
Title leukemiaRNA_TCL1_2
Sample type RNA
 
Source name TCL1 transgenic mouse leukemia RNA
Organism Mus musculus
Characteristics transgenic mice: TCL1 mouse
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from leukemia cells using TRIzol® reagent (Life Technologies,Carlsbad, CA) according to the manufacturer’s protocol followed by Turbo DNase treatment (Life Technologies, Carlsbad, CA) to remove trace amounts of genomic DNA.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the Ambion WT Expression Kit (Applied Biosystems) and then fragmented and labeled with the GeneChip WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the UCSD GeneChip Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description RMA expression value derived from BioConductor Oligo package
Data processing Raw data were processed with the BioConductor Oligo package for background correction and normalization. Data analysis and statistical evaluations were performed in R (version 2.15.1, R Core Team (2012). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.r-project.org/).
 
Submission date Oct 18, 2013
Last update date Jan 02, 2014
Contact name Emanuela Maria Ghia
E-mail(s) eghia@health.ucsd.edu
Organization name University of California San Diego
Department Center For Novel Therapeutics
Street address 9310 Athena Circle
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL6193
Series (1)
GSE51420 ROR1 Can Interact With TCL1 And Enhance Leukemogenesis in Eµ-TCL1 Transgenic Mice

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
4304920 1.26758897616559
4304921 0.706312439342752
4304922 6.96179601786764
4304923 4.44848000517353
4304925 3.81825262713205
4304927 1.9765302335415
4304928 6.98579632123694
4304929 9.56185592196625
4304930 5.28527614973656
4304931 2.32182488715776
4304932 3.37495506802465
4304933 4.1723517158028
4304934 2.03560150811212
4304935 1.81968646368786
4304937 2.75535672399929
4304938 6.00816601290794
4304939 1.72122939915072
4304940 3.48439304627058
4304941 2.71158436413214
4304942 7.33838525514853

Total number of rows: 1236087

Table truncated, full table size 30084 Kbytes.




Supplementary file Size Download File type/resource
GSM1245166_13278_320-+.CEL.gz 19.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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