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Sample GSM1245959 Query DataSets for GSM1245959
Status Public on Jan 03, 2014
Title IL-4 20 ng/ml
Sample type RNA
 
Source name monocyte-derived macrophages
Organism Homo sapiens
Characteristics culture condition: IL-4 20 ng/ml
Treatment protocol At day 7 cells were treated for 24 hours with Dexamethasone 100nM, LPS 10 ng/ml, IL-10 20 ng/ml, IL-13 20 ng/ml, IL-4 20 ng/ml or IFNy 20 ng/ml
Growth protocol Monocytes were isolated from buffy coats of healthy blood donors by Ficoll and percoll density gradientsand culture in X Vivo-10 supplemented with 1% Autologous serum for 7 days.
Extracted molecule total RNA
Extraction protocol Rneasy Total RNA isolation
Label biotin
Label protocol Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion, Inc., Austin, TX, U.S.A.) according to the manufacturer’s specifications with an input of 200 ng total RNA.
 
Hybridization protocol Hybridization and washing were performed according to the Illumina Manual “Direct Hybridization Assay Guide”
Scan protocol Scanning was performed on the Illumina iScan (Illumina, Inc., San Diego, CA, U.S.A.).
Description 1% autologous serum macrophages
Data processing Image analysis and extraction of raw expression data was performed with Illumina GenomeStudio v2011.1 Gene Expression software with default settings (no background substraction and no normalization)
 
Submission date Oct 20, 2013
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL10558
Series (2)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
GSE51446 Similarities and differences between human activated macrophages II

Data table header descriptions
ID_REF
VALUE RMA
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 6.558440813 0.4662338
ILMN_2055271 6.778449961 0.02337662
ILMN_1736007 6.579857063 0.3896104
ILMN_2383229 6.513936251 0.6467533
ILMN_1806310 6.520975212 0.6142857
ILMN_1779670 6.474220482 0.7766234
ILMN_1653355 6.688735715 0.1025974
ILMN_1717783 6.405269153 0.9155844
ILMN_1705025 6.475510826 0.7727273
ILMN_1814316 6.474877619 0.7766234
ILMN_2359168 6.423596945 0.887013
ILMN_1731507 6.415322862 0.9025974
ILMN_1787689 6.597046441 0.3428572
ILMN_3241953 7.031523737 0.001298701
ILMN_1745607 11.22377979 0
ILMN_2136495 6.49803573 0.6987013
ILMN_1668111 6.489360054 0.7194805
ILMN_2295559 6.635386353 0.2246753
ILMN_1735045 6.670645469 0.1337662
ILMN_1680754 6.555395901 0.4844156

Total number of rows: 47225

Table truncated, full table size 1538 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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