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Sample GSM1246576 Query DataSets for GSM1246576
Status Public on Sep 19, 2015
Title Human primary hepatocytes donor 2 infected with Ad-GFP
Sample type RNA
 
Source name Human primary hepatocytes donor 2 infected with Ad-GFP
Organism Homo sapiens
Characteristics tissue of origin: Liver
cell type: primary hepatocytes
genotype/variation: Ad-GFP
Treatment protocol Adenoviruses were added directly to the culture medium and cells were collected 24hr later. For experiments involving siRNA transfetions, cells were transfected with the indicated siRNAs 48hr prior to infection with adenoviruses encoding either GFP ("Ad-GFP") or PGC-1alpha ("Ad-PGC-1alpha").
Growth protocol Human primary hepatocytes were supplied in Hepatocyte medium (BD). HepG2 cells were grown in EMEM supplemented with 10% FBS. C2C12 cells were grown in DMEM with 20% FBS, and differentiated in DMEM containing 2% donor equine serum and 1uM insulin for 7 days.
Extracted molecule total RNA
Extraction protocol Using the Rneasy kit from Qiagen according to manufacturer's protocol
Label biotin
Label protocol Three hundred ng of total RNA was processed for cRNA synthesis with the Ambion WT Expression Kit (PN4411974). Fragmentation and terminal labeling was peformed with the AffymetrixGeneChip WT Terminal Labeling Kit (PN 900671)
 
Hybridization protocol Hybridization was performed at 45 degrees celcius for 17 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
Scan protocol The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from human primary hepatocytes donor 2 infected with Ad-GFP
Data processing The data expressed as CEL files were normalized by the robust multiarray average (RMA) method with the Expression Console software (Affymetrix).
 
Submission date Oct 21, 2013
Last update date Sep 19, 2015
Contact name Neri Minsky
E-mail(s) nminsky@rockefeller.edu
Organization name Rockefeller University
Lab Laboratory of Biochemistry and Molecular Biology
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6244
Series (1)
GSE51498 Regulation of HSF1-mediated transcriptional programs by PGC-1alpha

Data table header descriptions
ID_REF
VALUE log2 of RMA probe set intensity

Data table
ID_REF VALUE
8075838 5.654496
8095380 5.149235
8095364 5.163318
7936050 6.840868
7893190 6.782247
8037079 6.614177
8106689 7.131891
8141843 6.084992
8111677 8.140783
7912145 5.923238
7968637 6.277543
8055872 6.526616
7905220 8.855928
8063590 8.585591
8029086 5.036674
8179617 7.125844
8124707 6.8573
8178330 7.079675
8114938 5.054627
8060604 9.181549

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1246576_hHep_donor2_GFP.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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