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Sample GSM1246583 Query DataSets for GSM1246583
Status Public on Sep 19, 2015
Title Human primary hepatocytes donor 4 infected with Ad-PGC-1alpha
Sample type RNA
 
Source name Human primary hepatocytes donor 4 infected with Ad-PGC-1alpha
Organism Homo sapiens
Characteristics tissue of origin: Liver
cell type: primary hepatocytes
genotype/variation: Ad-PGC-1alpha
Treatment protocol Adenoviruses were added directly to the culture medium and cells were collected 24hr later. For experiments involving siRNA transfetions, cells were transfected with the indicated siRNAs 48hr prior to infection with adenoviruses encoding either GFP ("Ad-GFP") or PGC-1alpha ("Ad-PGC-1alpha").
Growth protocol Human primary hepatocytes were supplied in Hepatocyte medium (BD). HepG2 cells were grown in EMEM supplemented with 10% FBS. C2C12 cells were grown in DMEM with 20% FBS, and differentiated in DMEM containing 2% donor equine serum and 1uM insulin for 7 days.
Extracted molecule total RNA
Extraction protocol Using the Rneasy kit from Qiagen according to manufacturer's protocol
Label biotin
Label protocol Three hundred ng of total RNA was processed for cRNA synthesis with the Ambion WT Expression Kit (PN4411974). Fragmentation and terminal labeling was peformed with the AffymetrixGeneChip WT Terminal Labeling Kit (PN 900671)
 
Hybridization protocol Hybridization was performed at 45 degrees celcius for 17 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
Scan protocol The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from Human primary hepatocytes donor 4 infected with Ad-PGC-1alpha
Data processing The data expressed as CEL files were normalized by the robust multiarray average (RMA) method with the Expression Console software (Affymetrix).
 
Submission date Oct 21, 2013
Last update date Sep 19, 2015
Contact name Neri Minsky
E-mail(s) nminsky@rockefeller.edu
Organization name Rockefeller University
Lab Laboratory of Biochemistry and Molecular Biology
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6244
Series (1)
GSE51498 Regulation of HSF1-mediated transcriptional programs by PGC-1alpha

Data table header descriptions
ID_REF
VALUE log2 of RMA probe set intensity

Data table
ID_REF VALUE
8075838 11.60736
8095380 11.19791
8095364 11.1816
7936050 9.360559
7893190 9.984972
8037079 11.6471
8106689 12.18957
8141843 10.99032
8111677 11.66179
7912145 11.15446
7968637 11.52104
8055872 10.96555
7905220 12.79681
8063590 11.30602
8029086 8.154538
8179617 6.310204
8124707 6.09655
8178330 6.465478
8114938 6.222559
8060604 9.993114

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1246583_hHep_donor4_PGC.CEL.gz 4.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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