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Sample GSM1246586 Query DataSets for GSM1246586
Status Public on Sep 19, 2015
Title HepG2 cells infected with Ad-PGC-1alpha
Sample type RNA
 
Source name HepG2 cells infected with Ad-PGC-1alpha
Organism Homo sapiens
Characteristics tissue of origin: Liver
cell type: HepG2 cells
genotype/variation: Ad-PGC-1alpha
Treatment protocol Adenoviruses were added directly to the culture medium and cells were collected 24hr later. For experiments involving siRNA transfetions, cells were transfected with the indicated siRNAs 48hr prior to infection with adenoviruses encoding either GFP ("Ad-GFP") or PGC-1alpha ("Ad-PGC-1alpha").
Growth protocol Human primary hepatocytes were supplied in Hepatocyte medium (BD). HepG2 cells were grown in EMEM supplemented with 10% FBS. C2C12 cells were grown in DMEM with 20% FBS, and differentiated in DMEM containing 2% donor equine serum and 1uM insulin for 7 days.
Extracted molecule total RNA
Extraction protocol Using the Rneasy kit from Qiagen according to manufacturer's protocol
Label biotin
Label protocol Three hundred ng of total RNA was processed for cRNA synthesis with the Ambion WT Expression Kit (PN4411974). Fragmentation and terminal labeling was peformed with the AffymetrixGeneChip WT Terminal Labeling Kit (PN 900671)
 
Hybridization protocol Hybridization was performed at 45 degrees celcius for 17 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
Scan protocol The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from HepG2 cells infected with Ad-PGC-1alpha
Data processing The data expressed as CEL files were normalized by the robust multiarray average (RMA) method with the Expression Console software (Affymetrix).
 
Submission date Oct 21, 2013
Last update date Sep 19, 2015
Contact name Neri Minsky
E-mail(s) nminsky@rockefeller.edu
Organization name Rockefeller University
Lab Laboratory of Biochemistry and Molecular Biology
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6244
Series (1)
GSE51498 Regulation of HSF1-mediated transcriptional programs by PGC-1alpha

Data table header descriptions
ID_REF
VALUE log2 of RMA probe set intensity

Data table
ID_REF VALUE
7934979 7.721753
7906764 8.686544
8064978 8.476455
7951662 7.564327
8095728 6.825777
7956759 6.44195
8015133 7.148778
8095744 8.308351
8095736 7.856077
7927732 6.97622
8065071 6.335286
8180317 7.434551
7957140 7.423622
7906775 9.498755
8067140 7.386874
8180318 8.082018
8180316 8.106947
7958174 8.81789
8180319 7.618406
7917779 7.110819

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1246586_HepG2_PGC-1alpha.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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