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Sample GSM1246588 Query DataSets for GSM1246588
Status Public on Sep 19, 2015
Title HepG2 cells infected with SiControl Ad-GFP
Sample type RNA
 
Source name HepG2 cells infected with SiControl Ad-GFP
Organism Homo sapiens
Characteristics tissue of origin: Liver
cell type: HepG2 cells
genotype/variation: SiControl, Ad-GFP
Treatment protocol Adenoviruses were added directly to the culture medium and cells were collected 24hr later. For experiments involving siRNA transfetions, cells were transfected with the indicated siRNAs 48hr prior to infection with adenoviruses encoding either GFP ("Ad-GFP") or PGC-1alpha ("Ad-PGC-1alpha").
Growth protocol Human primary hepatocytes were supplied in Hepatocyte medium (BD). HepG2 cells were grown in EMEM supplemented with 10% FBS. C2C12 cells were grown in DMEM with 20% FBS, and differentiated in DMEM containing 2% donor equine serum and 1uM insulin for 7 days.
Extracted molecule total RNA
Extraction protocol Using the Rneasy kit from Qiagen according to manufacturer's protocol
Label biotin
Label protocol Three hundred ng of total RNA was processed for cRNA synthesis with the Ambion WT Expression Kit (PN4411974). Fragmentation and terminal labeling was peformed with the AffymetrixGeneChip WT Terminal Labeling Kit (PN 900671)
 
Hybridization protocol Hybridization was performed at 45 degrees celcius for 17 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
Scan protocol The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from HepG2 cells transfected with control siRNAs and then infected with Ad-GFP
Data processing The data expressed as CEL files were normalized by the robust multiarray average (RMA) method with the Expression Console software (Affymetrix).
 
Submission date Oct 21, 2013
Last update date Sep 19, 2015
Contact name Neri Minsky
E-mail(s) nminsky@rockefeller.edu
Organization name Rockefeller University
Lab Laboratory of Biochemistry and Molecular Biology
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6244
Series (1)
GSE51498 Regulation of HSF1-mediated transcriptional programs by PGC-1alpha

Data table header descriptions
ID_REF
VALUE log2 of RMA probe set intensity

Data table
ID_REF VALUE
7894664 6.271325
7894296 6.672242
7894731 6.40144
7893177 7.693396
7894570 4.39334
7893358 5.548096
7893902 6.170274
7893654 6.567037
7895250 6.658026
7894869 4.83988
7896406 6.533988
7896347 5.264129
7951662 10.08894
7893736 5.849298
7894196 6.721041
8084878 5.979024
7895992 5.895936
7893644 6.177154
7894901 6.675083
8037079 6.94135

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1246588_HepG2_SiControl_GFP.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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