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Sample GSM1246592 Query DataSets for GSM1246592
Status Public on Sep 19, 2015
Title C2C12 cells infected with Ad-GFP
Sample type RNA
 
Source name C2C12 cells infected with Ad-GFP
Organism Mus musculus
Characteristics tissue of origin: Muscle
cell type: C2C12 myotubes
genotype/variation: Ad-GFP
Treatment protocol Adenoviruses were added directly to the culture medium and cells were collected 24hr later. For experiments involving siRNA transfetions, cells were transfected with the indicated siRNAs 48hr prior to infection with adenoviruses encoding either GFP ("Ad-GFP") or PGC-1alpha ("Ad-PGC-1alpha").
Growth protocol Human primary hepatocytes were supplied in Hepatocyte medium (BD). HepG2 cells were grown in EMEM supplemented with 10% FBS. C2C12 cells were grown in DMEM with 20% FBS, and differentiated in DMEM containing 2% donor equine serum and 1uM insulin for 7 days.
Extracted molecule total RNA
Extraction protocol Using the Rneasy kit from Qiagen according to manufacturer's protocol
Label biotin
Label protocol Three hundred ng of total RNA was processed for cRNA synthesis with the Ambion WT Expression Kit (PN4411974). Fragmentation and terminal labeling was peformed with the AffymetrixGeneChip WT Terminal Labeling Kit (PN 900671)
 
Hybridization protocol Hybridization was performed at 45 degrees celcius for 17 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
Scan protocol The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from C2C12 myotubes infected with Ad-GFP
Data processing The data expressed as CEL files were normalized by the robust multiarray average (RMA) method with the Expression Console software (Affymetrix).
 
Submission date Oct 21, 2013
Last update date Sep 19, 2015
Contact name Neri Minsky
E-mail(s) nminsky@rockefeller.edu
Organization name Rockefeller University
Lab Laboratory of Biochemistry and Molecular Biology
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6246
Series (1)
GSE51498 Regulation of HSF1-mediated transcriptional programs by PGC-1alpha

Data table header descriptions
ID_REF
VALUE log2 of RMA probe set intensity

Data table
ID_REF VALUE
10563597 9.682408
10450369 12.31471
10450367 13.21701
10444589 9.278451
10520452 8.447759
10452316 9.781042
10358476 9.647501
10462442 9.327936
10379511 9.606948
10523120 8.190259
10379228 9.126679
10496592 8.300962
10519497 7.665402
10496555 7.397327
10368638 7.622801
10379518 9.052968
10489463 8.433163
10339171 4.884117
10505438 7.275878
10398121 10.10242

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM1246592_C2C12_GFP.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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