|
| Status |
Public on Oct 23, 2013 |
| Title |
Smoke_testis rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Adult testis from cigarette smoke exposed mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: adult
exposed to: cigarette smoke in utero
tissue: testis
|
| Treatment protocol |
C57BL/6 5 week-old female mice were exposed via the nose-only to cigarette smoke [twelve 3R4F reference cigarettes (University of Kentucky, USA) twice/day, five times per week, for 12-18 weeks]. Each exposure lasted 60 minutes. Control mice received room air. In total, 27 mice underwent cigarette smoke exposure. Eleven week-old female mice exposed to cigarette smoke for 6 weeks were separated into groups of three and housed with a single control stud male aged 7-8 weeks with proven fertility for a maximum of 12 weeks. Females were monitored every second day for post-coital plugs and pregnancy. Pregnant females were separated into single cages and litter sizes/pup weights recorded. Smoke exposure via dams continued throughout mating/pregnancy/lactation until weaning of pups at 21days post birth.
|
| Growth protocol |
All experimental procedures involving the use of animals were performed with the approval of the University of Newcastle’s Animal Care and Ethics Committee (ACEC). Specific pathogen-free adult female (6-week-old) C57BL/6 mice were obtained from the animal services unit and maintained according to the recommendations prescribed by the ACEC. Mice were housed under a controlled lighting regime (16L:8D) at 21–22°C and supplied with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (approximately 3 µg) was isolated from adult ovaries using a QIAGEN RNeasy Mini Kit (Hilden, Germany) in accordance with the manufacturer’s recommendations.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Smoke 2
|
| Data processing |
The data were normalised using quantile normalisation using BioConductor (v.2.12.)'s lumi package in R statistical software
|
| |
|
| Submission date |
Oct 22, 2013 |
| Last update date |
Oct 23, 2013 |
| Contact name |
Alexander Peter Sobinoff |
| E-mail(s) |
c3045119@uon.edu.au
|
| Organization name |
University of Newcastle Australia
|
| Department |
Science and IT
|
| Lab |
Reproductive Science Group
|
| Street address |
University Drive
|
| City |
Newcastle |
| State/province |
NSW |
| ZIP/Postal code |
2308 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE51530 |
Expression data from adult mouse testis exposed to cigarette smoke in utero |
|
