|
| Status |
Public on Nov 01, 2013 |
| Title |
Colon tissue: SIV infected, untreated macaque biological rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
SIV infected, untreated macaque colon tissue
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: colonic mucosa
age: 4-6 years
|
| Treatment protocol |
One SIV infected macaque (30378) was administered antiretroviral therapy beginning at 6 weeks post-infection, and two SIV infected macaques (33162 and 34540) were administered antiretroviral therapy beginning at 10 weeks post-infection
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard protocols described in the Affymetrix Gene Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rhesus macaque 3' IVT arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from SIV infected, untreated macaque colon tissue
|
| Data processing |
The data were analyzed with GeneSpring software, version 9
|
| |
|
| Submission date |
Oct 23, 2013 |
| Last update date |
Nov 01, 2013 |
| Contact name |
Michael George |
| E-mail(s) |
mdgeorge@ucdavis.edu
|
| Organization name |
University of California, Davis
|
| Department |
Medical Microbiology and Immunology
|
| Street address |
451 Health Sciences Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE51615 |
Expression data from rhesus macaque colon, jejunum, and lung |
|
