C57BL6/J mice (2 males) were sacrificed and cerebella were quickly removed. Total RNA was isolated using Trizol. For the creation of the library, 40 micrograms of total RNA was used. cDNA was synthesized on oligo dT beads. Enzyme: NlaIII Vector: pZERO-1 Restriction sites 1 & 2: Sph1 Library treatment: non-normalized