|
| Status |
Public on Oct 29, 2013 |
| Title |
mES 2 |
| Sample type |
RNA |
| |
|
| Source name |
mES
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mES
cell line: D3
|
| Treatment protocol |
untreated for each sample condition
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA with RNeasy Mini kit with DNase I digestion according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip human Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Description |
mES
|
| Data processing |
the data were RMA normalized and analysed with Partek Genomic Suit
|
| |
|
| Submission date |
Oct 28, 2013 |
| Last update date |
Oct 29, 2013 |
| Contact name |
christophe desterke |
| E-mail(s) |
christophe.desterke@inserm.fr
|
| Phone |
+33 (0)145595316
|
| Organization name |
university Paris Sud11
|
| Department |
UFR medecine institute Andre Lwoff
|
| Lab |
hopital Paul Brousse Batiment Lavoisier
|
| Street address |
14-16 avenue Paul Vaillant Couturier
|
| City |
VILLEJUIF |
| ZIP/Postal code |
94807 |
| Country |
France |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE51782 |
Gene expression profiles of C57BL/6 murine hepatocytes, murine embryonic stem cells (D3) and murine hepatocyte-reprogrammed induced Tissue Stem cells (iTSCs) |
|
