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Sample GSM1252991 Query DataSets for GSM1252991
Status Public on Nov 09, 2013
Title LPS 12hrs
Sample type RNA
 
Source name 24hrs with 1ug/ml LPS treatment
Organism Mus musculus
Characteristics background strain: C57BL6
cell type: JAWS II dendritic cells
Treatment protocol JAWS II dendritic cells (10^7 cells/culture in 10 ml plates) were incubated with 0.001% W805EC NE alone or mixed with 10 µg/ml rPA. Control cultures were either untreated (PBS) or incubated with 10 µg/ml rPA or with 1 µg/ml of TLR4 ligand LPS K12
Growth protocol Jaws II cells were grown according ATCC protocol
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated aRNA were prepared according to the Affymetrix GeneChip 3’ IVT Express kit protocol from 250 ng total RNA (Affymetrix GeneChip 3’ IVT Express kit Instruction Manual, P/N 702646 Rev. 5).
 
Hybridization protocol Following fragmentation, 16 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
Description Global gene expression in vitro
Data processing Data was analyzed using the limma and affy bioconductor packages implemented in the R-statistical environment version 2.15.0. RMA was used to normalize the data and fit log2 transformed expression values.
 
Submission date Oct 28, 2013
Last update date Nov 09, 2013
Contact name Paul Edward Makidon
E-mail(s) pmakidon@gmail.com
Phone 734-277-5775
Organization name University of Michigan
Department Internal Medicine
Lab MNIMBS
Street address 9340 MSRB III, 1150 W. Medical Center Dr.
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platform ID GPL1261
Series (1)
GSE51804 Effect of W805EC nanoemulsion (NE) on global gene transcription in JawsII dendritic cells in vitro

Data table header descriptions
ID_REF
VALUE Log 2 Transformed RMA

Data table
ID_REF VALUE
1415670_at 10.620
1415671_at 12.649
1415672_at 12.405
1415673_at 9.050
1415674_a_at 10.166
1415675_at 9.964
1415676_a_at 12.368
1415677_at 11.462
1415678_at 10.910
1415679_at 12.476
1415680_at 11.395
1415681_at 11.563
1415682_at 9.275
1415683_at 12.565
1415684_at 10.478
1415685_at 9.969
1415686_at 10.940
1415687_a_at 13.551
1415688_at 11.309
1415689_s_at 9.734

Total number of rows: 45101

Table truncated, full table size 772 Kbytes.




Supplementary file Size Download File type/resource
GSM1252991_Baker_Jr_010_138_Mouse430_2_.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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