|
| Status |
Public on Nov 01, 2013 |
| Title |
Islet Progenitors at Day 0 of differentiation, Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreas
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
developmental stage: Day 0 of differentiation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality check performed with Agilent Bioanalyser 2100
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA amplification kit from Ambion
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Scanning setting: 1.6 for Beadarray reader
|
| Data processing |
The data was normalised using RSN (Robust Spline Method). The software used is ' R ' Statistical language and environment.Module Lumi in Bioconductor release 2.13 within R was used for Normalization.
|
| |
|
| Submission date |
Oct 31, 2013 |
| Last update date |
Nov 04, 2013 |
| Contact name |
Munish Mehta |
| E-mail(s) |
munish.mehta@uwa.edu.au
|
| Phone |
+61-8-92240397
|
| Organization name |
Harry Perkin Institute of Medical Reasearch
|
| Department |
Diabetes Research Unit
|
| Lab |
DRF
|
| Street address |
L6, Rear 50 Murray Street
|
| City |
Perth |
| State/province |
WA |
| ZIP/Postal code |
6000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE51944 |
Robust Differentiation of Islet Progenitors into Functional Insulin-secreting Cells by Suppression of Key Transcription Regulators |
|
