|
| Status |
Public on Dec 22, 2014 |
| Title |
WT_1 |
| Sample type |
RNA |
| |
|
| Source name |
Livers of WT mice sacrificed at 4 weeks
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
genotype: TAK1 flox
strain: C57BL/6
tissue: liver
age: 4 wk
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Mice were maintained at all times on a 12-hour light-dark cycle in a specific pathogen-free environment with free access to rodent chow and water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Oct 31, 2013 |
| Last update date |
Dec 22, 2014 |
| Contact name |
Ekihiro Seki |
| E-mail(s) |
Ekihiro.Seki@cshs.org
|
| Phone |
310-423-6605
|
| Organization name |
Cedars-Sinai Medical Center
|
| Department |
Medicine
|
| Street address |
8700 Beverly Blvd., DAVIS, Suite 2099
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE51962 |
Gene expression profile of WT and TAK1 KO livers. |
|
