|
| Status |
Public on Nov 14, 2013 |
| Title |
WT Treg |
| Sample type |
RNA |
| |
|
| Source name |
WT B6 spleen
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
genetic background: C57BL/6
cell type: CD4+ CD25+ GITR+ (Treg) cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy RNA extraction kit (Qiagen) was used.
|
| Label |
biotin-16-UTP
|
| Label protocol |
Labeling procedures were carred out according to the standard protocol from Illumina.
|
| |
|
| Hybridization protocol |
hybridization and wash were done according to the Illumina's manual. (Hyb:Incubation at 58c for at least 14 hours)
|
| Scan protocol |
by Illumina iScan
|
| Description |
Gene expression
male, WT B6, 6 week old
|
| Data processing |
GeneSpring
|
| |
|
| Submission date |
Nov 13, 2013 |
| Last update date |
Nov 14, 2013 |
| Contact name |
Eui Ho Kim |
| E-mail(s) |
ekim38@wisc.edu
|
| Phone |
608-263-4715
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pathobiological Sciences
|
| Lab |
Dr. M. Suresh
|
| Street address |
2015 Linden Dr.
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE52337 |
mRNA expression in CD4+ CD25+ GITR+ regulatory T cells from WT and Bach2 KO mice |
|
