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Sample GSM126437 Query DataSets for GSM126437
Status Public on Jul 31, 2008
Title mammary gland-1mg progesterone intraperitoneally-biologic replicate 1 MG-U74B
Sample type RNA
 
Source name mammary gland
Organism Mus musculus
Characteristics eight week old virgin BALB/cJ mice were ovariectomized followed by a period of one week before treatment to clear endogenous hormones
lymph node-free mammary gland
Treatment protocol ovariectomized animals were injected daily with 1 mg progesterone for 4 days
Extracted molecule total RNA
Extraction protocol Ultraspec for RNA extraction (Biotexc, Houston, TX)
Label biotin
Label protocol Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U74A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500.
Description Mammary gland mRNA expression data from mice treated with progesterone.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Aug 09, 2006
Last update date Dec 27, 2007
Contact name D. Joseph Jerry
E-mail(s) jjerry@vasci.umass.edu
Phone 413-545-5335
Fax 413-545-6326
URL http://htpp://www.umass.edu/vasci/faculty/jerry/jerry.html
Organization name University of Massachusetts
Department Veterinary & Animal Scineces
Street address 161 Holdsworth Way
City Amherst
State/province MA
ZIP/Postal code 01003
Country USA
 
Platform ID GPL82
Series (1)
GSE5483 Transcriptional Profiling of Responses to Estrogen and Progesterone in Mammary Gland

Data table header descriptions
ID_REF Affymetrix probeset identifier
VALUE MAS5 calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or Marginal (M).
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
104769_at 33.4 A 0.07383
104770_at 30.6 A 0.5
104771_at 16.9 A 0.837065
104772_at 143.6 A 0.098054
104773_at 14.1 A 0.47938
104774_at 38.5 A 0.081337
104775_at 236.7 P 0.035163
104776_at 72.8 A 0.339558
104777_at 7.2 A 0.715253
104778_at 76 A 0.060419
104779_at 118.5 A 0.378184
104780_at 99.9 A 0.234557
104781_at 29.5 A 0.284747
104782_at 283.3 P 0.013092
104783_at 27.3 A 0.765443
104784_at 83 M 0.048995
104785_at 92.8 M 0.048995
104786_at 37.8 A 0.267463
104787_at 107.8 A 0.438361
104788_at 111.4 A 0.234557

Total number of rows: 12477

Table truncated, full table size 320 Kbytes.




Supplementary data files not provided

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